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Test Number : NS0-002
Test Name : NetApp Certified Technology Associate
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NS0-002 test Format | NS0-002 Course Contents | NS0-002 Course Outline | NS0-002 test Syllabus | NS0-002 test Objectives

You demonstrate a foundational understanding of NetApp� data storage systems and the products and technologies specifically designed for managing data in todays growing hybrid cloud market. You understand technologies in storage and data management, both on-premise and in a hybrid cloud environment and have an in-depth knowledge of NetApp products and technologies used in managing data in the cloud.

NCTA will be granted to those individuals who successfully pass the NetApp Certified Technology Associate (NS0-002) exam.
The NetApp Certified Technology Associate (NS0-002) test includes the following topics:
Infrastructure Concepts
Identify basic infrastructure, governance and virtualization concepts
NetApp Data Storage Software
Identify NetApp Element, NetApp ONTAP, NetApp StorageGRID, NetApp SANtricity
NetApp Cloud Solutions
Describe NetApp administration tools
Describe NetApp data mobility solutions
Identify NetApp cloud data protection
Describe NetApp Cloud Storage
NetApp Hybrid Cloud Value Proposition
Describe the business benefits of the NetApp Data Fabric
Identify the consumption model options for NetApp Hybrid Cloud solutions

NetApp Certified Technology Associate (NS0-002)
� AWS website
� Build your Data Fabric with NetApp HCI, ONTAP and Converged Infrastructure TR-4748
� Docker website
� Google Cloud website
� Kubernetes Documentation site
� National Institute of Standards and Technology (NIST)
� NetApp Blog
� NetApp Cloud Blog
� NetApp Cloud Central
� NetApp Cloud Documentation
� NetApp Datasheet
� NetApp Forums
� NetApp Information Center
� NetApp IO
� NetApp Knowledgebase
� NetApp Kubernetes Hub
� NetApp Library
� NetApp MySupport
� NetApp Product Documentation
� NetApp Product Page
� NetApp Solution Brief
� NetApp StorageGRID Documentation
� NetApp Technical Report
� NetApp website
� NetApp White Paper
� VMware

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Identification of an unauthorized genetically modified bacteria in meals enzyme via total-genome sequencing | NS0-002 Practice Questions and test dumps

A meals enzyme (protease) product commercialized on the eu market, used in this study, was currently suspected to be contaminated by using unauthorized GMM. On the one hand, starting from DNA extracted from the meals enzyme matrix, the presence of bacterial DNA belonging to the Bacillus genus became tested through PCR amplification and Sanger sequencing of the bacterial 16 S rRNA gene region8 (Fig. 1 step 1). in addition, the abilities GM nature of this infection changed into suspected in line with a positive precise-time PCR sign for the aminoglycoside adenyltransferase (aadD) gene conferring a resistance to each kanamycin (KanR) and neomycine (NeoR) (GenBank: M19465.1) (Fig. 1 step 1)10. The presence of the entire-size dimension of this aadD gene turned into subsequently confirmed through nested-PCR mixed with Sanger sequencing, highlighting competencies fitness and environmental dangers associated to this food enzyme product in mild of AMR acquisition concerns9,10. even so, importantly, a achievable bacterial strain became remoted from the validated food enzyme product8. This bacterial pressure became subjected to the same evaluation as above applied on DNA extracted from the food enzyme matrix. in a similar fashion to the outcomes followed using DNA extracted from the food enzyme matrix, this bacterial pressure turned into shown to belong to the Bacillus genus8 in addition to to lift the aadD gene (Fig. 1 step 2, table 1, Supplementary file 1). The presence of a possible GM Bacillus strain in the proven pattern changed into due to this fact strongly suspected, but, these outcomes have been insufficient to without doubt show the presence of this GMM and signify its associated genetic modifications.

figure 1

Schematic representation of the workflow, composed of three main successive steps, applied on the validated food enzyme product, permitting to determine unauthorized GMM by means of total-genome sequencing (WGS) and subsequently to increase event-particular true-time PCR methods. (1) DNA extracted from the FE practise was established for the presence of bacterial DNA as well as the presence of AMR genes frequently harboured through GMM used to supply FE. (2) residing microbial traces, prior isolated from the FE guidance, were tested for the presence of bacterial DNA and subsequent determination of their genus/species in addition to for the presence of AMR genes commonly harboured by means of GMM used to supply FE. (three) The bacterial traces identified in (2) as carrying AMR genes turned into characterised by means of a WGS strategy the use of a de novo assembly analysis with the intention to demonstrate the presence of a possible unauthorized GMM in the established FE instruction. With the generated sequences, true-time PCR methods particular to this GMM had been developed for use by enforcement laboratories.

desk 1 Oligonucleotides used for PCR-primarily based strategies. GMM identification the use of WGS

the use of an Illumina MiSeq gadget (250 bp paired-conclusion reads), WGS applied on DNA from the bacterial strain isolated from the food enzyme product (Fig. 1 step 3, Supplementary file 2) generated 714,637 paired-conclusion raw reads. Following examine trimming, 589,817 splendid reads (ordinary Phred score of 37) had been retained to perform a de novo meeting, enabling to generate 430 contiguous sequences (contigs) of which forty seven have been longer than 1,000 bases with a ok-mer coverage of as a minimum 10x. Contig sizes ranged from 56 bp to 457,195 bp, with an N50 value of 291,658.

On the one hand, the generated contigs introduced a correspondence to the Bacillus genus, and, more exactly, notably to the B. velezensis species (RefSeq: NZ_CP011937.1) in its place of the expected B. subtilis species that turned into labelled as being the producer organism of the commercialized neutral protease. This identification become based on three observations. at first, when the use of the meeting for typing in opposition t the B. subtilis MLST schema hosted via the PubMLST.org internet-based mostly platform, an ideal match to sequence type a hundred and forty was bought for which best a single isolate become latest in the database (PubMLST: ATCC 12321) annotated because the species B. velezensis. Secondly, a k-mer based classification of sequencing reads towards an in-house dump of all finished genomes in the RefSeq Microbial Genomes database indicated the presence of B. velezensis (Supplementary file three). Thirdly, this identification changed into proven by means of performing a examine mapping analysis to the NCBI representative B. velezensis reference genome sequence (RefSeq: NZ_CP001937.1), with a median depth and breadth of coverage of respectively 58x and ninety four.58% (Supplementary file four). B. velezensis species is not listed by using EFSA (2018) as getting used in the meals and feed business to produce meals and feed components, enzymes and flavourings intended for the ecu market30. despite the fact, this species, for which the wild-classification is innocent for human and carefully involving B. amyloliquefaciens and B. subtilis, has previously been described as tremendously helpful for producing enzymes, together with proteases, for the agro-industrial sector31,32,33,34,35,36.

having said that, the generated contigs had been blasted against the aadD gene, conferring KanR and NeoR, that was prior detected through actual-time PCR in addition to nested-PCR adopted by Sanger sequencing analysis10 (table 1, Supplementary file 1). A contig of 349,285 bp with a okay-mer insurance of fifty nine.434x turned into identified as harbouring the focused AMR gene (Fig. 2, Supplementary file 5). to be able to determine the putative transgenic insertion, the regions flanking this AMR gene have been then characterized and in comparison to the reference genome of B. velezensis (RefSeq: NZ_CP011937.1). in the reference genome, a region of two,385 bp from place 2,460,164 to 2,462,548, with >ninety nine% sequence identification, composed of a gene coding for a protease (GenPept: WP_032874795.1; RS12020 in Fig. 2) as well as part of a gene coding for an acetyltransferase (GenPept: WP_032874793.1; RS12025 in Fig. 2), became changed by using a fraction of 9,141 bp in the genome of the remoted bacterial strain containing the place of two,385 bp in reproduction. given that the confirmed meals enzyme product changed into commercialized as a protease, the duplication of this location can for this reason be defined by using the purpose of the producers to increase protease yield right through the creation method. Between these duplicated areas, a chain of 4,102 bp, with a question coverage and identification of one hundred%, matching to the pUB110 shuttle vector (GenBank: M19465.1) harbouring the aadD gene (GenBank: AAA88361.1), conferring KanR and NeoR, that turned into earlier recognized by means of real-time PCR and nested-PCR (Fig. 1), and the ble gene (RefSeq: NG_047557.1), conferring a resistance to bleomycin (BleoR) changed into characterised (Fig. 2, Supplementary file 5). This pUB110 shuttle vector, originating from Staphylococcus aureus, and the identified AMR genes had been up to now mentioned as being totally used in GMM producing bacterial fermentation products within the food and feed chain, principally for alternative of traces of interest10,37. additionally, the accompanied left and right transgene flanking areas of the inserted fragment of 9,141 bp as well because the left and appropriate transgene flanking regions of the pUB110 shuttle vector had been proven through PCR adopted by Sanger sequencing (Supplementary info 5,6). in accordance with all these outcomes, the presence of a genetic change specific to a potential GMM within the commercialized food enzyme product became for this reason verified. These results, communicated to the Belgian Federal company for the safeguard of the meals Chain, have led to the RASFF 2019.3332 notification at the european degree.

determine 2

Schematic illustration of the identified transgenic insertion. The pUB110 shuttle vector (eco-friendly) harbours the aadD gene conferring a resistance to kanamycin (KanR) (crimson) and the ble gene conferring a resistance to bleomycin (BleoR) (yellow). Blue rectangles symbolize annotated genes on the reference genome. The region indicated in orange contains a gene coding for a protease (RS12020) and a part of a gene coding for an acetyltransferase (RS12025). The latter, indicated by using a small darkish crimson rectangle within the GM consists out of a full (RS12025) and interrupted (RS12025a) replica. The red area is wonderful within the wild-classification while this crimson vicinity is duplicated, on both sides of the pUB110 shuttle vector, in the GMM. The darkish and hatched rectangles point out the areas targeted with the aid of the left (L) and appropriate (R) adventure-selected true-time PCR methods developed and validated in this study.

concerning the bioinformatics methodology, compared to a read-mapping analysis, a de novo assembly evaluation was probably the most central strategy to determine and symbolize an unknown and unauthorized GMM for 2 explanations. in the beginning, no reference sequence is required, representing an potential e.g. in the latest analyze because of the unavailability of a reference sequence for the identified GMM. This approach is additionally useful when the species id of the GMM host is difficultly identifiable, as exemplified during this examine with the Bacillus strain8. Secondly, via reconstructing a contig containing the transgenic insertion in the wild-category B. velezensis genome, an unnatural association of sequence facets may well be inferred, proposing robust facts of the presence of a GMM. with out an attainable reference sequence for a particular GMM, a read-mapping evaluation cannot supply this category of essential tips. indeed, most effective the presence of sequences belonging both to the pUB110 shuttle vector or to B. velezensis might then had been proven, however no hyperlink between the pUB110 shuttle vector and B. velezensis might have been based (Supplementary file 4).

development of experience-specific true-time PCR strategies in line with WGS statistics

in response to characterization of the transgenic insertion into B. velezensis, two adventure-specific real-time PCR strategies have been developed and validated, permitting to especially goal cost- and time-effectively the unauthorized GMM found in the present analyze (Fig. 1 step three). These two experience-selected strategies have been designed to cowl either the left or the right transgene flanking vicinity of the inserted pUB110 shuttle vector (Fig. 2; table 1; Supplementary file 5). For each and every real-time PCR method, an amplicon with the anticipated measurement and sequence turned into acquired (Supplementary file 7). The efficiency of those precise-time PCR strategies become then investigated.

First, the specificity of those real-time PCR methods changed into Tested the usage of, as superb control, DNA from the remoted GM B. velezensis RASFF 2019.3332 pressure as well as, as bad controls, DNA from eighty-5 wild-category microbial strains commonly used to produce microbial fermentation products9,10, DNA from six distinct wild-class B. velezensis lines, DNA from the nutrition B2-producing GM B. subtilis RASFF 2014.1249 strain, DNA from plant (Zea mays) and DNA from human. As anticipated, these event-selected precise-time PCR strategies offered a good sign most effective for the nice handle, confirming their specificity (table 2). 2d, the sensitivity of those real-time PCR strategies became assessed the usage of DNA from the GM B. velezensis RASFF 2019.3332 strain at distinct estimated full genome copy numbers (6 × 106, 6 × 104, 6 × 102, 60, 12, 6, 1, 0.1 and nil) (desk 3). For each true-time PCR strategies, a positive sign was accompanied at as little as one estimated full genome copy, demonstrating their high sensitivity. Third, the applicability of these precise-time PCR methods changed into proven using DNA from the commercialized meals enzyme product through which the GM B. velezensis RASFF 2019.3332 stress (pattern n°1) changed into detected as well as a commercialized nutrition B2 feed additive product (RASFF 2014.1249) (pattern n°2). As expected, both actual-time PCR strategies offered a positive signal for the pattern n°1 and a terrible signal for the trial n°2 (Supplementary file 6). in keeping with all these outcomes, both proposed event-selected precise-time PCR methods were evaluated as selected, sensitive and relevant, permitting enforcement laboratories to effectively goal the GM B. velezensis RASFF 2019.3332 pressure in commercialized microbial fermentation items. If necessary, following to extra optimisation and validation steps, these real-time PCR strategies may well be mixed into a duplex assay.

table 2 list of wild-classification microorganisms used for specificity assessment of the true-time PCR strategies. The presence and absence of amplification are respectively symbolized by “+” and “-”. For every result, the scan turned into conducted in duplicate. DNA from the GM Bacillus subtilis (RASFF 2014.1249) stress, ninety-one wild-category microbial lines, plant and animal have been used as terrible control. DNA from the GM Bacillus velezensis (RASFF 2019.332) strain became used as wonderful handle. table three Sensitivity assessment of precise-time PCR methods. For each confirmed DNA concentration from the GM Bacillus velezensis RASFF 2019.3332 pressure, the corresponding estimated full genome reproduction quantity is indicated. The presence and absence of amplification are respectively symbolized through “+” and “−”. For each and every effect at each DNA awareness, the experiment changed into conducted in quadruplicate. From 25 to 0.0000025 ng, each and every replicate generated a favorable signal. The capability of the accompanied Cq are indicated between brackets. From 0.00000025 to 0 ng, each replicate generated a poor sign.

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Lotus [66 Certification Exam(s) ]
LPI [25 Certification Exam(s) ]
LSI [3 Certification Exam(s) ]
Magento [3 Certification Exam(s) ]
Maintenance [1 Certification Exam(s) ]
McAfee [9 Certification Exam(s) ]
McData [3 Certification Exam(s) ]
Medical [68 Certification Exam(s) ]
Microsoft [408 Certification Exam(s) ]
Mile2 [3 Certification Exam(s) ]
Military [1 Certification Exam(s) ]
Misc [2 Certification Exam(s) ]
Motorola [7 Certification Exam(s) ]
MuleSoft [1 Certification Exam(s) ]
mySQL [4 Certification Exam(s) ]
NBSTSA [1 Certification Exam(s) ]
NCEES [2 Certification Exam(s) ]
NCIDQ [1 Certification Exam(s) ]
NCLEX [3 Certification Exam(s) ]
Network-General [12 Certification Exam(s) ]
NetworkAppliance [42 Certification Exam(s) ]
NetworkAppliances [1 Certification Exam(s) ]
NI [1 Certification Exam(s) ]
NIELIT [1 Certification Exam(s) ]
Nokia [8 Certification Exam(s) ]
Nortel [130 Certification Exam(s) ]
Novell [38 Certification Exam(s) ]
OMG [10 Certification Exam(s) ]
Oracle [317 Certification Exam(s) ]
P&C [2 Certification Exam(s) ]
Palo-Alto [6 Certification Exam(s) ]
PARCC [1 Certification Exam(s) ]
PayPal [1 Certification Exam(s) ]
PCI-Security [1 Certification Exam(s) ]
Pegasystems [19 Certification Exam(s) ]
PEOPLECERT [4 Certification Exam(s) ]
PMI [16 Certification Exam(s) ]
Polycom [2 Certification Exam(s) ]
PostgreSQL-CE [1 Certification Exam(s) ]
Prince2 [7 Certification Exam(s) ]
PRMIA [2 Certification Exam(s) ]
PsychCorp [1 Certification Exam(s) ]
PTCB [2 Certification Exam(s) ]
QAI [1 Certification Exam(s) ]
Qlik [2 Certification Exam(s) ]
QlikView [2 Certification Exam(s) ]
Quality-Assurance [6 Certification Exam(s) ]
RACC [1 Certification Exam(s) ]
Real-Estate [2 Certification Exam(s) ]
RedHat [8 Certification Exam(s) ]
RES [5 Certification Exam(s) ]
Riverbed [9 Certification Exam(s) ]
RSA [16 Certification Exam(s) ]
Sair [8 Certification Exam(s) ]
Salesforce [10 Certification Exam(s) ]
SANS [2 Certification Exam(s) ]
SAP [98 Certification Exam(s) ]
SASInstitute [15 Certification Exam(s) ]
SAT [2 Certification Exam(s) ]
SCO [10 Certification Exam(s) ]
SCP [6 Certification Exam(s) ]
SDI [3 Certification Exam(s) ]
See-Beyond [1 Certification Exam(s) ]
ServiceNow [1 Certification Exam(s) ]
Siemens [1 Certification Exam(s) ]
Snia [7 Certification Exam(s) ]
SOA [15 Certification Exam(s) ]
Social-Work-Board [4 Certification Exam(s) ]
Splunk [3 Certification Exam(s) ]
SpringSource [1 Certification Exam(s) ]
SUN [63 Certification Exam(s) ]
SUSE [1 Certification Exam(s) ]
Sybase [17 Certification Exam(s) ]
Symantec [137 Certification Exam(s) ]
Teacher-Certification [4 Certification Exam(s) ]
The-Open-Group [8 Certification Exam(s) ]
TIA [3 Certification Exam(s) ]
Tibco [18 Certification Exam(s) ]
Trainers [3 Certification Exam(s) ]
Trend [1 Certification Exam(s) ]
TruSecure [1 Certification Exam(s) ]
USMLE [1 Certification Exam(s) ]
VCE [7 Certification Exam(s) ]
Veeam [2 Certification Exam(s) ]
Veritas [34 Certification Exam(s) ]
Vmware [76 Certification Exam(s) ]
Watchguard [1 Certification Exam(s) ]
Wonderlic [2 Certification Exam(s) ]
Worldatwork [3 Certification Exam(s) ]
XML-Master [3 Certification Exam(s) ]
Zend [6 Certification Exam(s) ]

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